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The Automated Fluorescence
Imaging System (AFIS) is a total digital solution to your critical
fluorescence requirements. The system includes all the hardware and
software to control your automated microscope and create exceptional
deconvolved and 3D images.
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Click on the image for animation
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Digital Camera
- Roper Scientifics' CoolSnap
hq, a 12 bit monochrome camera with a resolution of 1392 x 1040
pixels and exceptional sensitivity in all wavelengths.
Computer - The
latest Intel processor with 1 GB of RAM, 17" flat panel LCD
display, 60 GB hard drive, DVD Burner, CD-RW and Windows 2000. |
Software - Media
Cybernetic's Image-Pro Plus the
industries leading image analysis software, Scope-Pro
for precise control of your computer automated microscope, SharpStack
allows you to create deconvolved images from your stack of fluorescence
images, 3D Constructor will
build a 3 dimensional model of the deconvolved image stack and AFA
(Advance Fluorescence Acquisition) for the management and control of
all your image stacks. |
System
Description
Image-Pro -
controls the image acquistion, exposure times, gain and binning capabilities
of the CoolSnap hq. Image-Pro also implements the
automated particle counting, measurements, processing and image enhancements.
More info...
Scope-Pro -
controls the automated mechanics of the microscope by adjusting the focus
for acquiring multiple 'Z' planes, changing the shutter to reduce
photo-bleaching, moving the x and y location of the stage for tiling
multiple images together and changing objective for high magnification. More
info...

Pig cerebellum image deconvolved using the inverse filter
algorithm in SharpStack
SharpStack
- The budget conscious Confocal microscope alternative. This takes the 'Z'
stack created with Scope-Pro and removes the "haze" and out of
focus artifacts present in fluorescence images. See example above. More
info...
3D Constructor
- Generate 3 dimensional images of the deconvolved 'Z' stack images. 3D
Constructor gives you the capability to create 3D animations and save the
animation as an AVI file for playback in PowerPoint, Windows Media Player
or QuickTime. Click on the 3D image above to see a sample of the animation
playback capability. More info...
AFA -
Advanced Fluorescence Acquisition is the image manager and nerve center
for the Scope-Pro and SharpStack. AFA keeps the image location and other
relevant information required to sift through the quantitative data
and provide qualitative results. More
info...
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System Requirements for AFIS
Supported Microscopes
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Olympus AX Series, BX2 Series, IX-81,
IX2 Series |
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Leica DM RXA, DM IRBE, DM RXA2, DM RA2,
DM LA2, DM IRE2, M-Series Stereo |
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Nikon E1000, E1000M, TE2000 |
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Zeiss AxioPlan 2, Axiovert |
Supported Stages, Filters
and Shutters
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Ludl BioPoint controller and stages |
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Ludl MAC 2000/2002/5000 controller for
stages, filter wheels, and shutters |
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Prior H128/129
series controller and stages |
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Prior Optiscan
controller and stages |
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Märzhäuser MultiControl 2000
controller and stages |
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Märzhäuser L-Step controller and
stages |
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Sutter Lambda 10-2 and 10-C filter
wheels and shutters |
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Vincent Associates shutters |
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Nikon Remote Focus Motor attachment |
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Eco-Step X, Y stage controller (no Z) |
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Zeiss MCU-26 X, Y stage |
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For further information, or a
product demonstration, please feel free to contact
IPS.
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